Composite
Part:BBa_K4171023:Design
Designed by: HUANG, YEN-JUNG Group: iGEM22_NCKU_Tainan (2022-08-16)
Ptrc-gadB-B0015
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 739
Illegal EcoRI site found at 1177 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 739
Illegal EcoRI site found at 1177 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 739
Illegal EcoRI site found at 1177
Illegal BamHI site found at 654 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 739
Illegal EcoRI site found at 1177 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 739
Illegal EcoRI site found at 1177
Illegal NgoMIV site found at 1305
Illegal AgeI site found at 567 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
None
Source
Trc promoter: BBa_K864400
gadB: BBa_M50089
Terminator: BBa_B0015