Composite

Part:BBa_K4171023:Design

Designed by: HUANG, YEN-JUNG   Group: iGEM22_NCKU_Tainan   (2022-08-16)


Ptrc-gadB-B0015


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 739
    Illegal EcoRI site found at 1177
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 739
    Illegal EcoRI site found at 1177
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 739
    Illegal EcoRI site found at 1177
    Illegal BamHI site found at 654
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 739
    Illegal EcoRI site found at 1177
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 739
    Illegal EcoRI site found at 1177
    Illegal NgoMIV site found at 1305
    Illegal AgeI site found at 567
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Trc promoter: BBa_K864400

gadB: BBa_M50089

Terminator: BBa_B0015

References